教員の研究紹介
黒澤 信幸

生体情報システム科学専攻　Major of Biological Information Systems

• TEL : 076-445-6982
• URL : http://pse.eng.u-toyama.ac.jp/life/
• Keywords : gene engineering, molecular biology

研究の背景と目的　Background and Purpose of Study

免疫は、感染症からの防御やがんの制御などを担っており、その制御に関わる獲得免疫系では、抗体が中心的な働きをしています。ヒト、ウサギ、モルモット、ラットなどの様々な動物の単一形質細胞からモノクローナル抗体を迅速に開発する技術を開発することで、各種疾患に対する治療用、診断用、研究用抗体の取得およびその改変を行なっています。

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Immunity plays a crucial role in defending the body against infectious diseases and controlling cancer. Antibodies are central to the acquired immune system and are essential for this defense. By developing technologies to rapidly produce monoclonal antibodies from individual plasma cells of various animals, including humans, rabbits, guinea pigs, and rats, we are able to acquire and modify therapeutic, diagnostic, and research antibodies to combat various diseases.Immunity is responsible for protecting against infectious diseases and controlling cancer, and antibodies play a central role in the acquired immune system involved in such control. By developing technologies to rapidly develop monoclonal antibodies from single plasma cells of various animals, including humans, rabbits, guinea pigs, and rats, we are acquiring and modifying therapeutic, diagnostic, and research antibodies for various diseases.

本研究の領域横断性

我々の研究室では微量試料からのハイスループットな遺伝子解析・発現システムを開発しており、遺伝子工学をベースとした物作りに協力することができると考える。

参考文献

• Comprehensive analysis of nasal IgA antibodies induced by intranasal administration of the SARS-CoV-2 spike protein Kentarou Waki, Hideki Tani, Yumiko Saga, Takahisa Shimada, Emiko Yamazaki, Seiichi Koike, Okada Mana, Masaharu Isobe, Nobuyuki Kurosawa elife, 2023, Nov 7, 2023  Peer-reviewedLast authorCorresponding author
• The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through membrane immunoassay device. Kengo Nishimura, Hiroaki Kitazawa, Takashi Kawahata, Kosuke Yuhara, Takahiro Masuya, Toshihiro Kuroita, Kentarou Waki, Seiichi Koike, Masaharu Isobe, Nobuyuki Kurosawa BMC immunology, 24(1) 34-34, Sep 26, 2023
• Novel super-neutralizing antibody UT28K is capable of protecting against infection from a wide variety of SARS-CoV-2 variants Tatsuhiko Ozawa, Hideki Tani, Yuki Anraku, Shunsuke Kita, Emiko Igarashi, Yumiko Saga, Noriko Inasaki, Hitoshi Kawasuji, Hiroshi Yamada, so Ichiro Sasaki … mAbs, 14(1) e2072455, Apr 27, 2022  Peer-reviewed
• A candidate gene of Alzheimer diseases was mutated in senescence-accelerated mouse prone (SAMP) 8 mice. Maruf Mohammad Akbor, Juhyon Kim, Mai Nomura, Juno Sugioka, Nobuyuki Kurosawa, Masaharu Isobe Biochemical and biophysical research communications, 572 112-117, Aug 4, 2021
• Development of a T-cell receptor mimic antibody targeting a novel Wilms tumor 1-derived peptide and analysis of its specificity. Nobuyuki Kurosawa, Aki Midorikawa, Kenta Ida, Yuka Wakata Fudaba, Masaharu Isobe Cancer science, 111(10) 3516-3526, Oct, 2020
• High throughput development of TCR-mimic antibody that targets survivin-2B80-88/HLA-A*A24 and its application in a bispecific T-cell engager. Nobuyuki Kurosawa, Yuka Wakata, Kenta Ida, Aki Midorikawa, Masaharu Isobe Scientific reports, 9(1) 9827-9827, Jul 8, 2019  Peer-reviewed
• Development and validation of monoclonal antibodies against N6-methyladenosine for the detection of RNA modifications. Shun Matsuzawa, Yuka Wakata, Fumiya Ebi, Masaharu Isobe, Nobuyuki Kurosawa PloS one, 14(10) e0223197, 2019  Peer-reviewed
• Guinea pig immunoglobulin VH and VL naïve repertoire analysis. Shun Matsuzawa, Masaharu Isobe, Nobuyuki Kurosawa PloS one, 13(12) e0208977, 2018  Peer-reviewed
• Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies Nobuyuki Kurosawa, Yuka Wakata, Tomonao Inobe, Haruki Kitamura, Megumi Yoshioka, Shun Matsuzawa, Yoshihiro Kishi, Masaharu Isobe SCIENTIFIC REPORTS, 6 25174, Apr, 2016  Peer-reviewed
• Possible involvement of Hcn1 ion channel in learning and memory dysfunction in SAMP8 mice. Maruf Mohammad Akbor, Koji Tomobe, Tomomi Yamada, Juhyon Kim, Hiroki Mano, Nobuyuki Kurosawa, Kazuo Sasaki, Yasuyuki Nomura, Masaharu Isobe Biochemical and biophysical research communications, 441(1) 25-30, Nov 8, 2013  Peer-reviewed
• Reduced Level of the BCL11B Protein Is Associated with Adult T-Cell Leukemia/Lymphoma Nobuyuki Kurosawa, Rika Fujimoto, Tatsuhiko Ozawa, Takahiro Itoyama, Naoki Sadamori, Masaharu Isobe PLOS ONE, 8(1) e55147, Jan, 2013  Peer-reviewed